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As a result of their lipophilicity and chemical stability, PCBs have been detected all over the world in remote regions, PCBs have attracted considerable attention due to their ability to concentrate in food chains and their persistence in organisms.During the past few decades, there has been a decline of organism PCBs levels associated with the production ban on PCBs.One class of persistent organic pollutant, polychlorinated biphenyls (PCBs), was originally manufactured for industrial applications due to its insulating and flame-retardant properties.Several recent studies on PCBs have found that they possess endocrine disrupting activity.Despite previous findings, the data on the PCB load in human sperm are lacking in terms of studies on the effects of PCBs on the male human reproduction system.The purpose of the present study was to analyze the changes in motility parameters, mitochondrial membrane potential, and intracellular reactive oxygen species (ROS) levels of human sperm treated with Aroclor 1254 for an exhaustive toxicological assessment of this PCB mixture.The motility of 200 spermatozoa was evaluated by CASA.The sperm motility parameters were evaluated after 3 and 6 h of incubation with 0, 1, 5, or 25 mg l Aroclor 1254.
Toxic effects of polychlorinated biphenyls (Aroclor 1254) on human sperm motility.
The sperm suspensions were incubated for 3 and 6 h at 37°C in a humidified atmosphere of 5% CO), beat cross frequency (BCF, Hz), linearity (LIN, %), and straightness (STR, %).
An aliquot of 10 μl of each sample was transferred to a Makler chamber that had been prewarmed to 37°C.
To this aim, a short-term in vitro incubation experiment that better imitated the putative exposure of sperm to Aroclor 1254 (a commercial PCB mixture) in male reproduction tissue was conducted.
Human sperm were incubated with various concentrations (0, 1, 5, or 25 mg l) of Aroclor 1254 for different amounts of time (3 and 6 h) in vitro.
The relative ROS production was calculated as the ratio of the MFI of the treated sample to the MFI of the vehicle control.